ywbA [2019-08-26 11:34:56]

similar to [category|SW 1.2.2|PTS], putative EIIC component

locus: BSU_38390

pI: 7.89

mw: 47.48 kDa

protein length: 444 aa

gene length: 1335 bp

function: unknown

product: unknown

essential: no

synonyms: ipa-16d

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,938,307 3,939,641

The protein

Protein family

• [category|SW 1.2.2|PTS], lactose EIIC family [Pubmed|10627040]

Paralogous protein(s)

• [protein|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|GmuC], [protein|83A7C160B0301A2B51B65478D99ACB753D67AF74|LicC]

[SW|Domains]

• [SW|PTS EIIC domain] type-3 (aa 116-455) (according to UniProt)

Structure

• [PDB|3QNQ] (EIIC component of diacetylchitobiose specific PTS from ''Bacillus cereus''; 60% identity, 87% similarity) [Pubmed|21471968]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|ywbA]-[gene|64E4F910148F9349FA68FC685310D14C0A72BE8B|ywbB]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE38390 ([gene|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|ywbA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAAAAACCCCCTTTT, downstream forward: _UP4_TGATAGATGATCAGCCTGTC
• BKK38390 ([gene|0650C9EAB395EB6ABDADBE72F0DA9F8E5E606B0E|ywbA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAAAAACCCCCTTTT, downstream forward: _UP4_TGATAGATGATCAGCCTGTC

References

• 10627040